Cytotoxicity of carbamide peroxide bleaching gel on L929 cells

نویسندگان

  • Rogério Lacerda dos Santos
  • Matheus Melo Pithon
  • Fernanda Otaviano Martins
  • Maria Teresa Villela Romanos
چکیده

Methods: Three concentrations of carbamide peroxide (10%, 16%, and 22%) used in home bleaching techniques were evaluated regarding their cytotoxic effect on gingival tissues. The materials were divided into three groups as follows: Group C10 (White Gold Home 10%, Dentsply), Group C16 (White Gold Home 16%, Dentsply, and Group C22 (Nite White 22%, ACP Discus Dental). The cytotoxicity essay was carried out using cell cultures (mouse fibroblast L929 cell line) in which the viable cells were determined by means of the dye-uptake method performed at 2, 4, and 8 hours. Data were analyzed by analysis of variance (ANOVA) with multiple comparisons and Tukey’s test (P<0.05).

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تاریخ انتشار 2010